LOCUS       V001886                 7410 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V001886
VERSION     V001886
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7410)
  AUTHORS   Trauth S, Bischofs IB.
  TITLE     Ectopic Integration Vectors for Generating Fluorescent Promoter
            Fusions in Bacillus subtilis with Minimal Dark Noise
  JOURNAL   PLoS ONE 9 (5), E98360 (2014)
   PUBMED   24874808
REFERENCE   2  (bases 1 to 7410)
  AUTHORS   Trauth S, Bischofs IB.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2014) Center for Molecular Biology and BioQuant,
            University of Heidelberg, Im Neuenheimer Feld 282, Heidelberg, BW
            69120, Germany
REFERENCE   3  (bases 1 to 7410)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7410)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE";
            date: "2014"; volume: "9"; issue: "5"; pages: "E98360"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (30-APR-2014) Center for Molecular Biology and BioQuant, University
            of Heidelberg, Im Neuenheimer Feld 282, Heidelberg, BW 69120,
            Germany"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7410
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        104..208
                     /label="AmpR promoter"
     CDS             209..1066
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      1240..1828
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     rep_origin      1829
                     /label="ColE1 origin of DNA replication (+1)"
                     /note="ColE1 origin of DNA replication (+1)"
     misc_feature    complement(2014..2154)
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     CDS             complement(2259..2447)
                     /label="rop"
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     misc_recomb     complement(3384..4579)
                     /label="homologous recombination site amyE back"
                     /note="homologous recombination site amyE back"
     CDS             complement(4952..5599)
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     misc_feature    5763..5779
                     /label="small cloning site (SCS)"
                     /note="small cloning site (SCS)"
     regulatory      5780..5840
                     /label="Bacillus subtilis gyrA transcriptional terminator"
                     /note="Bacillus subtilis gyrA transcriptional terminator"
                     /regulatory_class="terminator"
     stem_loop       5792..5812
                     /label="Bacillus subtilis gyrA transcriptional terminator"
                     /note="Bacillus subtilis gyrA transcriptional terminator"
     misc_feature    5845..5873
                     /label="LIC site"
                     /note="LIC site"
     CDS             5913..6629
                     /label="EYFP"
                     /note="enhanced YFP"
     primer_bind     complement(6634..6650)
                     /label="SK primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        complement(6683..6701)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6708..6724)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     misc_recomb     complement(6773..7410)
                     /label="homologous recombination site amyE front"
                     /note="homologous recombination site amyE front"
ORIGIN
        1 ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
       61 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
      121 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
      181 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
      241 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
      301 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
      361 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
      421 agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg
      481 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
      541 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
      601 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
      661 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
      721 accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact
      781 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
      841 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
      901 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
      961 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     1021 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     1081 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     1141 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     1201 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     1261 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     1321 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     1381 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     1441 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     1501 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     1561 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     1621 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     1681 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     1741 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     1801 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     1861 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     1921 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     1981 cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca
     2041 tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc
     2101 atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga
     2161 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
     2221 agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg
     2281 aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg
     2341 tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt
     2401 ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg
     2461 taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg
     2521 atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg
     2581 gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt
     2641 aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac
     2701 ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag
     2761 accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc
     2821 tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc
     2881 ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg
     2941 cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt
     3001 tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg
     3061 ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac
     3121 gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc
     3181 atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta
     3241 ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc
     3301 tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa
     3361 tggggaaggc catccagcct cgcggtatgt ttctctttga tgtctttttg tttgtgaagt
     3421 atttcacatt tatattgtgc aacacttcac aaacttttgc aagagaaaag ttttgtctga
     3481 tttatgaaca aaaaagaaac catcattgat ggtttctttc ggtaagtccc gtctagcctt
     3541 gccctcaatg gggaagagaa ccgcttaagc ccgagtcatt atataaacca tttagcacgt
     3601 aatcaaagcc aggctgattc tgaccgggca cttgggcgct gccattatta aaaatcactt
     3661 ttgcgttggt tgtatccgtg tccgcaggca gcgtcagcgt gtaaattccg tctgcatttt
     3721 tagtcattgg ttttccaggc caagatccgg tcaattcaat tactcggctc ccatcatgtt
     3781 tatagatata agcatttacc tggctccaat gattcggatt ttgatagccg atggttttgg
     3841 ccgacgctgg atctctttta acaaaactgt atttctcggt cctcgttaca ccatcactgt
     3901 tcgttccttt taacatgatg gtgtatgttt tgccaaatgg atctcctttt ccgattgtga
     3961 attgatctcc atccttaaac gccgtctctg gtccattatt gatttgataa acggcttttg
     4021 ttgtattcgc atctgcacgc aaggtaatcg tcagttgatc attgaaagaa tgtgttacac
     4081 ctgttttgta attctcaagg aaaacatgag gcgcttttgc aatatcatca ggataaagca
     4141 cagctacaga cctggcattg atcgtgcctg tcagtttacc atcgttcact tgaaatgaac
     4201 ccgctccagc tttattgtca tacctgccat caggcaattt tgttgccgta ttgatagaga
     4261 cagaggatga acctgcattt gccagcacaa cgccatgtga gccgcgctga ttcataaata
     4321 tctggttgtt tccattcggg ttcgagagtt cctcaggctg tccagccatc acattgtgaa
     4381 atctattgac cgcagtgata gcctgatctt caaataaagc actcccgcga tcgcctattt
     4441 ggcttttccc ccagatctgg ggaacctcac accatttccg cctccctcag gtctggaaaa
     4501 gaaaagaggc gtactgcctg aacgagaagc tatcaccgcc cagcctaaac ggatatcatc
     4561 atcgctcatc catgtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt
     4621 agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg
     4681 cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg
     4741 agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca
     4801 accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatctggagc
     4861 tgtaatataa aaaccttctt caactaacgg ggcaggttag tgacattaga aaaccgactg
     4921 taaaaagtac agtcggcatt atctcatatt ataaaagcca gtcattaggc ctatctgaca
     4981 attcctgaat agagttcata aacaatcctg catgataacc atcacaaaca gaatgatgta
     5041 cctgtaaaga tagcggtaaa tatattgaat tacctttatt aatgaatttt cctgctgtaa
     5101 taatgggtag aaggtaatta ctattattat tgatatttaa gttaaaccca gtaaatgaag
     5161 tccatggaat aatagaaaga gaaaaagcat tttcaggtat aggtgttttg ggaaacaatt
     5221 tccccgaacc attatatttc tctacatcag aaaggtataa atcataaaac tctttgaagt
     5281 cattctttac aggagtccaa ataccagaga atgttttaga tacaccatca aaaattgtat
     5341 aaagtggctc taacttatcc caataaccta actctccgtc gctattgtaa ccagttctaa
     5401 aagctgtatt tgagtttatc acccttgtca ctaagaaaat aaatgcaggg taaaatttat
     5461 atccttcttg ttttatgttt cggtataaaa cactaatatc aatttctgtg gttatactaa
     5521 aagtcgtttg ttggttcaaa taatgattaa atatctcttt tctcttccaa ttgtctaaat
     5581 caattttatt aaagttcatt tgatatgcct cctaaatttt tatctaaagt gaatttagga
     5641 ggcttacttg tctgctttct tcattagaat caatcctttt ttaaaagtca atattactgt
     5701 aacataaata tatattttaa aaatatccca ctttatccaa ttttcgaatt cttttaccgc
     5761 ggggtaccat cgtggatccg tgtgaaaaaa agcgcagctg aaatagctgc gcttttttgt
     5821 gtcataaccc tttacagtca ttttaccgcg ggctttcccg ggaaggagga actactatgg
     5881 attcaataga aaaggtaagc gaatttgcca ccatggtgag caagggcgag gagctgttca
     5941 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg
     6001 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca
     6061 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccttcggc tacggcctgc
     6121 agtgcttcgc ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc
     6181 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc
     6241 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg
     6301 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca
     6361 acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc
     6421 acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg
     6481 gcgacggccc cgtgctgctg cccgacaacc actacctgag ctaccagtcc gccctgagca
     6541 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga
     6601 tcactctcgg catggacgag ctgtacaagt aaagatccac tagttctaga gcggccgcca
     6661 ccgcggtgga gctccaattc gccctatagt gagtcgtatt acaattcact ggccgtcgtt
     6721 ttacaacgtc gtgactggga aaaccgaatt cgaattccca ttatgtacta tttcgatcag
     6781 accagttttt aatttgtgtg tttccatgtg tccagtttgg aatactctta acctcattgg
     6841 aaatcgcggc ataatcactg gtggtatgat tgatgaccgc gtcaacaatg acctttatgc
     6901 catattcttc agcggctgca cacatttctt taaattcttg ttcagtacct aagtaacggt
     6961 tgccaatttg atacgatgtc ggctgataca gccagtacca gttcgacatg cttttatctc
     7021 cttgattccc ttcctttact tggttaatcg gagatgtctg aatggctgta tatcctgcat
     7081 catgaatatc cttcatattg tgttttaacg tattgaacga ccaattccat gcatgaagaa
     7141 tggttccgct tttgatcgac ggtgctgtaa gctcattcga tttgttcgcc gtttcagcac
     7201 tcgcagccgc cggtcctgcc agaaccaaat gaaacagcaa taaaaatcca gcgaataacg
     7261 gcagtaaaga ggttttgaat cgttttgcaa acattcttga cactccttat ttgatttttt
     7321 gaagacttac ttcggagtca aaaatccctc ttacttcatt cttccgcttc ctcctttcaa
     7381 accgatgtga agactggaga attttgttaa
//
